Biospecimen Collection

Benefits

Preparation

Comparisons

Evidence quality

Highlighted claims

• Blood samples are collected for DNA, genotyping, RNA sequencing, serum, plasma, and peripheral blood mononuclear cells. — Real-world and Genomic data-based Asthma Insights through Network Analysis (REGAIN study): protocol for a novel retrospective and prospective longitudinal asthma cohort study
• Nasal epithelial cells are collected at every asthma study visit and once from healthy controls. — Real-world and Genomic data-based Asthma Insights through Network Analysis (REGAIN study): protocol for a novel retrospective and prospective longitudinal asthma cohort study
• Nasal brushing is central because it is minimally invasive, inexpensive, efficient, and suitable for repeated sampling. — Real-world and Genomic data-based Asthma Insights through Network Analysis (REGAIN study): protocol for a novel retrospective and prospective longitudinal asthma cohort study
• Biospecimens are stored and processed in batches to reduce batch effects. — Real-world and Genomic data-based Asthma Insights through Network Analysis (REGAIN study): protocol for a novel retrospective and prospective longitudinal asthma cohort study
• Prior work cited by the protocol found high concordance between nasal airway epithelial cells and lower-airway cells for non-ubiquitously expressed genes. — Real-world and Genomic data-based Asthma Insights through Network Analysis (REGAIN study): protocol for a novel retrospective and prospective longitudinal asthma cohort study
• The protocol presents sputum induction and bronchoscopy as more burdensome and less complete in large studies than nasal brushing. — Real-world and Genomic data-based Asthma Insights through Network Analysis (REGAIN study): protocol for a novel retrospective and prospective longitudinal asthma cohort study