Cell-Free DNA

Cross-source consensus on Cell-Free DNA from 1 sources and 3 claims.

1 sources · 3 claims

Uses

Plasma for cfDNA extraction is separated using two centrifugation steps to avoid post-collection lymphocyte lysis, which could artificially increase cfDNA. — ReFIT study (reversing frailty in transplantation): protocol for a longitudinal study to assess clinical and biomedical changes in frailty through kidney transplantation
Mitochondrial cfDNA is assessed using target sequences from mitochondrial and nuclear genomes by real-time quantitative PCR on a ViiA 7 system, tested in duplicate. — ReFIT study (reversing frailty in transplantation): protocol for a longitudinal study to assess clinical and biomedical changes in frailty through kidney transplantation
If cfDNA concentrations are at least 5 to 10 ng/mL, methylation capture screening may be used to infer tissue or cell origins. — ReFIT study (reversing frailty in transplantation): protocol for a longitudinal study to assess clinical and biomedical changes in frailty through kidney transplantation